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transfection human pulmonary artery smooth muscle cells  (ATCC)


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    ATCC transfection human pulmonary artery smooth muscle cells
    Effects of DNAzymes on PKC-ε protein levels in cultured cells. A: Western blots of hPASMCs. Representative Western blots for PKC-ε (top) or β-tubulin (bottom) from hPASMCs treated with liposome alone, wtDNAzyme, mDNAzyme, or sDNAzyme are shown. B: quantification of protein levels in hPASMCs. Western blots from 5 separate <t>transfection</t> experiments using hPASMCs were digitized, analyzed by densitometry using NIH Image, and normalized to liposome only treatment. Values are expressed as normalized averages ± SE. A Mann-Whitney U-test gave a P value of 0.016 for the wtDNAzyme-induced PKC-ε decrease compared with the other conditions. C: Western blots of A7r5 cells. Representative Western blots for PKC-ε (top) or β-tubulin (bottom) from rat A7r5 cells treated with liposome alone, wtDNAzyme, mDNAzyme, or sDNAzyme are shown. D: quantification of protein levels in A7r5 cells. Western blots from 3 separate transfection experiments using A7r5 cells were digitized, analyzed by densitometry using NIH Image, and normalized to liposome only treatment, as described in B. *P = 0.003 for PKC-ε levels in wtDNAzyme-treated cells vs. all other conditions by Mann-Whitney U-test.
    Transfection Human Pulmonary Artery Smooth Muscle Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 978 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transfection+human+pulmonary+artery+smooth+muscle+cells/pmc04400177-81-3-27?v=ATCC
    Average 96 stars, based on 978 article reviews
    transfection human pulmonary artery smooth muscle cells - by Bioz Stars, 2026-07
    96/100 stars

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    1) Product Images from "Electroporation-mediated delivery of catalytic oligodeoxynucleotides for manipulation of vascular gene expression"

    Article Title: Electroporation-mediated delivery of catalytic oligodeoxynucleotides for manipulation of vascular gene expression

    Journal: American journal of physiology. Heart and circulatory physiology

    doi: 10.1152/ajpheart.00350.2003

    Effects of DNAzymes on PKC-ε protein levels in cultured cells. A: Western blots of hPASMCs. Representative Western blots for PKC-ε (top) or β-tubulin (bottom) from hPASMCs treated with liposome alone, wtDNAzyme, mDNAzyme, or sDNAzyme are shown. B: quantification of protein levels in hPASMCs. Western blots from 5 separate transfection experiments using hPASMCs were digitized, analyzed by densitometry using NIH Image, and normalized to liposome only treatment. Values are expressed as normalized averages ± SE. A Mann-Whitney U-test gave a P value of 0.016 for the wtDNAzyme-induced PKC-ε decrease compared with the other conditions. C: Western blots of A7r5 cells. Representative Western blots for PKC-ε (top) or β-tubulin (bottom) from rat A7r5 cells treated with liposome alone, wtDNAzyme, mDNAzyme, or sDNAzyme are shown. D: quantification of protein levels in A7r5 cells. Western blots from 3 separate transfection experiments using A7r5 cells were digitized, analyzed by densitometry using NIH Image, and normalized to liposome only treatment, as described in B. *P = 0.003 for PKC-ε levels in wtDNAzyme-treated cells vs. all other conditions by Mann-Whitney U-test.
    Figure Legend Snippet: Effects of DNAzymes on PKC-ε protein levels in cultured cells. A: Western blots of hPASMCs. Representative Western blots for PKC-ε (top) or β-tubulin (bottom) from hPASMCs treated with liposome alone, wtDNAzyme, mDNAzyme, or sDNAzyme are shown. B: quantification of protein levels in hPASMCs. Western blots from 5 separate transfection experiments using hPASMCs were digitized, analyzed by densitometry using NIH Image, and normalized to liposome only treatment. Values are expressed as normalized averages ± SE. A Mann-Whitney U-test gave a P value of 0.016 for the wtDNAzyme-induced PKC-ε decrease compared with the other conditions. C: Western blots of A7r5 cells. Representative Western blots for PKC-ε (top) or β-tubulin (bottom) from rat A7r5 cells treated with liposome alone, wtDNAzyme, mDNAzyme, or sDNAzyme are shown. D: quantification of protein levels in A7r5 cells. Western blots from 3 separate transfection experiments using A7r5 cells were digitized, analyzed by densitometry using NIH Image, and normalized to liposome only treatment, as described in B. *P = 0.003 for PKC-ε levels in wtDNAzyme-treated cells vs. all other conditions by Mann-Whitney U-test.

    Techniques Used: Cell Culture, Western Blot, Transfection, MANN-WHITNEY



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    transfection human pulmonary artery smooth muscle cells  (ATCC)
    96
    ATCC transfection human pulmonary artery smooth muscle cells
    Effects of DNAzymes on PKC-ε protein levels in cultured cells. A: Western blots of hPASMCs. Representative Western blots for PKC-ε (top) or β-tubulin (bottom) from hPASMCs treated with liposome alone, wtDNAzyme, mDNAzyme, or sDNAzyme are shown. B: quantification of protein levels in hPASMCs. Western blots from 5 separate <t>transfection</t> experiments using hPASMCs were digitized, analyzed by densitometry using NIH Image, and normalized to liposome only treatment. Values are expressed as normalized averages ± SE. A Mann-Whitney U-test gave a P value of 0.016 for the wtDNAzyme-induced PKC-ε decrease compared with the other conditions. C: Western blots of A7r5 cells. Representative Western blots for PKC-ε (top) or β-tubulin (bottom) from rat A7r5 cells treated with liposome alone, wtDNAzyme, mDNAzyme, or sDNAzyme are shown. D: quantification of protein levels in A7r5 cells. Western blots from 3 separate transfection experiments using A7r5 cells were digitized, analyzed by densitometry using NIH Image, and normalized to liposome only treatment, as described in B. *P = 0.003 for PKC-ε levels in wtDNAzyme-treated cells vs. all other conditions by Mann-Whitney U-test.
    Transfection Human Pulmonary Artery Smooth Muscle Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transfection+human+pulmonary+artery+smooth+muscle+cells/pmc04400177-81-3-27?v=ATCC
    Average 96 stars, based on 1 article reviews
    transfection human pulmonary artery smooth muscle cells - by Bioz Stars, 2026-07
    96/100 stars
    		  Buy from Supplier

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    Effects of DNAzymes on PKC-ε protein levels in cultured cells. A: Western blots of hPASMCs. Representative Western blots for PKC-ε (top) or β-tubulin (bottom) from hPASMCs treated with liposome alone, wtDNAzyme, mDNAzyme, or sDNAzyme are shown. B: quantification of protein levels in hPASMCs. Western blots from 5 separate transfection experiments using hPASMCs were digitized, analyzed by densitometry using NIH Image, and normalized to liposome only treatment. Values are expressed as normalized averages ± SE. A Mann-Whitney U-test gave a P value of 0.016 for the wtDNAzyme-induced PKC-ε decrease compared with the other conditions. C: Western blots of A7r5 cells. Representative Western blots for PKC-ε (top) or β-tubulin (bottom) from rat A7r5 cells treated with liposome alone, wtDNAzyme, mDNAzyme, or sDNAzyme are shown. D: quantification of protein levels in A7r5 cells. Western blots from 3 separate transfection experiments using A7r5 cells were digitized, analyzed by densitometry using NIH Image, and normalized to liposome only treatment, as described in B. *P = 0.003 for PKC-ε levels in wtDNAzyme-treated cells vs. all other conditions by Mann-Whitney U-test.

    Journal: American journal of physiology. Heart and circulatory physiology

    Article Title: Electroporation-mediated delivery of catalytic oligodeoxynucleotides for manipulation of vascular gene expression

    doi: 10.1152/ajpheart.00350.2003

    Figure Lengend Snippet: Effects of DNAzymes on PKC-ε protein levels in cultured cells. A: Western blots of hPASMCs. Representative Western blots for PKC-ε (top) or β-tubulin (bottom) from hPASMCs treated with liposome alone, wtDNAzyme, mDNAzyme, or sDNAzyme are shown. B: quantification of protein levels in hPASMCs. Western blots from 5 separate transfection experiments using hPASMCs were digitized, analyzed by densitometry using NIH Image, and normalized to liposome only treatment. Values are expressed as normalized averages ± SE. A Mann-Whitney U-test gave a P value of 0.016 for the wtDNAzyme-induced PKC-ε decrease compared with the other conditions. C: Western blots of A7r5 cells. Representative Western blots for PKC-ε (top) or β-tubulin (bottom) from rat A7r5 cells treated with liposome alone, wtDNAzyme, mDNAzyme, or sDNAzyme are shown. D: quantification of protein levels in A7r5 cells. Western blots from 3 separate transfection experiments using A7r5 cells were digitized, analyzed by densitometry using NIH Image, and normalized to liposome only treatment, as described in B. *P = 0.003 for PKC-ε levels in wtDNAzyme-treated cells vs. all other conditions by Mann-Whitney U-test.

    Article Snippet: Cell culture and transfection Human pulmonary artery smooth muscle cells (hPASMC; a gift of Dr. Paul Babal, Department of Pharmacology, University of South Alabama) and A7r5 cells (ATCC No. CRL-1444) were maintained in 100-mm plates containing DMEM with 10% FBS, 1× Kanamycin, and 1× antimycotic solution (Life Technologies; Gaithersberg, MD) at 37°C and 5% CO 2 .

    Techniques: Cell Culture, Western Blot, Transfection, MANN-WHITNEY